Development of a lab-on-a-chip device for the manipulation of microparticles using AC-dielectrophoresis and acoustic methods

(Completed Project)



  • Barbaros Çetin
  • Bülent Özer (TOBB-ETU-ME)
  • Soheila Zeinali
  • Erdem Çağatay (TOBB-ETU-ME)
  • Süleyman Büyükkoçak (TOBB-ETU-ME)


TUBITAK, 3501 - National Young Researchers Career Development Program (CAREER) (112M102)

Project Duration:

Start Date: November 15th, 2012
Completion Date: January 15th, 2015


The objective of this project is to develop a lab-on-a-chip (LOC) device which can manipulate microparticles using dielectrophoretic (DEP) and acoustic methods. This device enables integrated particle wash, particle separation and particle concentration. The non-uniform electrical field inside the device will be generated by means of electrodes which are embedded inside the microchannel, the acoustic force on the other hand will be generated with piezoelectric elements embedded in the device. Particle separation will be achieved with the DEP force, and particle wash and particle concentration will be achieved with the acoustic force. The device will be designed to have stable, robust and highly repeatable manufacturability. For biological tests the device needs to be disposable, therefore the device will be aimed to have a low manufacturing cost. Moreover, high repeatability of the processes that will be run on the device and a high performance will be the goal of the project. For the fabrication of the LOC device both lithography-based micro/nanofabrication techniques and miniature-machining based fabrication techniques will be utilized.

Related Publications:

    Journal Papers

  • Çetin, B., Özer, M.B., Çağatay, E., Büyükkoçak, S. (2016). "An integrated acoustic and dielectrophoretic particle manipulation in a microfluidic device for particle wash and separation fabricated by mechanical machining", Biomicrofluidics, v.10 p.014112
    Full Text | DOI:

  • Zeinali, S., Çetin, B., Oliaei, S., Karpat, Y. (2015). "Fabrication of continuous flow microfluidics device with 3D electrode structures for high throughput DEP applications using mechanical machining", Electrophoresis, Special Issue on Dielectrophoresis, v.36(13) p.1432-1442
    Full Text | DOI: 10.1002/elps.201400486

  • Büyükkoçak, S., Özer, B., Çetin, B. (2014). "Numerical modeling of acoustophoretic particle separation for microfuildics", Microfluid Nanofluid, v.17(6) p.1025-1037
    Full Text | DOI: 10.1007/s10404-014-1398-7

  • Conference Papers

  • Çağatay, E., Çetin, B., Özer, B. (2015). "An integrated microfluidic device for particle wash and particle separation", 2nd Novel Fluidic Technologies and Applications Workshop, April 9-10, İzmir, Turkey

  • Zeinali, S., Cetin, B., Büyükkoçak, S., Özer, B. (2014). "Fabrication of microfluidic devices for dielectrophoretic and acoustophoretic applications using high-precision machining", 16th Int. Conference on Machine Design and Production (UMTIK-2014), Paper No: 43, June 30 - July 3, Izmir, Turkey
    Full Text

  • Çetin, B., Zeinali, S. (2014). "Modeling of on-chip (bio)particle separation and counting using 3D electrode structures", 4th Micro and Nano Flows Conference, September 7-10, University College London, UK
    Full Text

  • Büyükkoçak, S., Özer, B., Çetin, B. (2014). "Finite element modeling of micro-particle manipulation using ultrasonic standing waves", ASME 12th International Conference on Nanochannels, Microchannels and Minichannels, August 3-7, Chicago, IL, USA
    Full Text

  • Çetin, B., Büyükkoçak, S., Zeinali, S., Özer, B. (2013). "Simulation of an integrated microfluidic device for bioparticle wash, separation and concentration", Proc. ASME 2013 4th Microscale/Nanoscale Heat & Mass Transfer International Conference, December 11-14, Hong Kong, China
    DOI: 10.1115/MNHMT2013-22181

  • Book Chapters

  • Büyükkoçak, S., Özer, M.B., Çetin, B. (2015). "Microscale Acoustofluidics", Encyclopedia of Micro and Nanofluidics (2nd Edition), Springer, p.2149-2158
    Full Text | DOI: 10.1007/978-3-642-27758-0_1735-4

  • M.Sc. Theses

  • Soheila Zeinali (Advisor: Asst. Prof. Dr. Barbaros Çetin), (2014). "Microfluidic device with 3D electrode structure for high throughput dielectrophoretic applications", Bilkent University, Mechanical Engineering Department, Ankara, Turkey
    Full Text

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