Project

Development of a microfluidic device for particle counting and the application of the device for the determination of the total viable count and the number of somatic cells in reference milk sample

(Completed Project)

Groups:

People:

  • Barbaros Çetin
  • Göksel Durkaya (Atılım Universityt- MSE)
  • Duygu Kışla (Ege Uni.-FE)
  • Gülten Gündüz (Ege Uni.-FE)
  • Reza Rasooli
  • Gamze Düven (Ege Uni.-FE)

Funding:

TÜBİTAK 1001 (114M597)

Project Duration:

Start Date: November 1st, 2014
Completion Date: March 1st, 2017
 

Summary:

The objective of this project is to develop a microfluidic device which can count live bacteria and somatic cells in a whole milk. For this purpose, both counting following the focusing of particles via inertial microfluidics, and counting via florescent tagging and without any separation are performed. The focusing of the particles wth diameter larger than 6 micrometer is achieved via inertial microfluidics. A computational model which predicts the focusing performance of a microfluidic device has been developed in the project. Due to the fact that optic signal intensity is not high enough for the whole milk applications, the counting is achieved with lower flow rates. A microfluidic device which enables counting of cells/bacteria at small flow rates has been designed and two alternative counting algorithms have been developed. For most of the bio-particle concentrations, the counting is achieved with 90% accuracy. Cell and bacteria count is performed via total 20 minutes of proses time on a portable platform. The counting device designed in this project has an overall dimension of 25cmx25cmx25cm and weights approximately 9 kg. The cost of the counting for one single set is estimated as 20TL.

Related Publications:

    Journal Papers

  • Düven, G., Çetin, B., Kışla, D. (2017). "Çip-üstü-laboratuvar teknolojisinin gıda mikrobiyolojisindeki uygulamaları (Lab-on-a-chip technology in food microbiology)", Akademik Gıda (Academic Food) (In press)

  • Conference Papers

  • Çetin, B., Kaplan, H., Durkaya, G., Kurtuldu, H. (2016). "Low cost, ultra high throughput particle counting using inertial microfluidics", The 20th Int. Conf. Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), October 9-13, Dublin, Ireland
    Full Text

  • Rasooli, R., Karaoğlu, O.K., Çetin, B. (2016). "Simulation of particle flow in a spiral microchannel for inertial microfluidics", 5th Micro and Nano Flows Conference, September 11-14, Milan, Italy
    Full Text

  • M.Sc. Theses

  • Reza Rasooli (Advisor: Asst. Prof. Dr. Barbaros Çetin), (2017). "Modeling of inertial particle flow and entry gas flow in micro-channels", Bilkent University, Mechanical Engineering Department, Ankara, Turkey

  • Gamze Düven (Co-supervisor: Asst. Prof. Dr. Barbaros Çetin), (2017). "Implementation of a microfluidic device for the determination of microbial load of milk", Ege University, Department of Food Engineering, İzmir, Turkey

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