DEVELOPMENT OF A MICROFLUIDIC DEVICE FOR PARTICLE COUNTING AND THE APPLICATION OF THE DEVICE FOR THE DETERMINATION OF THE TOTAL VIABLE COUNT AND THE NUMBER OF SOMATIC CELLS IN REFERENCE MILK SAMPLE

Tamamlanmış Proje

TÜBİTAK 1001 (114M597)

PROJECT DURATION

Start Date: November 1st, 2014
Completion Date: March 1st, 2017

SUMMARY

The objective of this project is to develop a microfluidic device which can count live bacteria and somatic cells in a whole milk. For this purpose, both counting following the focusing of particles via inertial microfluidics, and counting via florescent tagging and without any separation are performed. The focusing of the particles wth diameter larger than 6 micrometer is achieved via inertial microfluidics. A computational model which predicts the focusing performance of a microfluidic device has been developed in the project. Due to the fact that optic signal intensity is not high enough for the whole milk applications, the counting is achieved with lower flow rates. A microfluidic device which enables counting of cells/bacteria at small flow rates has been designed and two alternative counting algorithms have been developed. For most of the bio-particle concentrations, the counting is achieved with 90% accuracy. Cell and bacteria count is performed via total 20 minutes of proses time on a portable platform. The counting device designed in this project has an overall dimension of 25cmx25cmx25cm and weights approximately 9 kg. The cost of the counting for one single set is estimated as 20TL.

RELATED PUBLICATIONS

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  • Sabuncu, A.C., Çetin, B., Usta, O.B., Aubry, N. (2021). "β-dispersion of blood during sedimentation.", Scientific Reports, v.11(1) p.2642, Full Text
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  • Çetin, B., Kuşcu, Y.F., Çetin, B., Tümüklü, Ö., Cole, K.D. (2021). "Semi-analytical source (SAS) method for 3-D transient heat conduction problems with moving heat source.", Int. J. Heat Mass Trans., v.165 p.120692, Full Text
  • Akkuş, Y., Çetin, B., Dursunkaya, Z. (2020). "A theoretical framework for comprehensive modeling of steadily fed evaporating droplets and the validity of common assumptions", Int. J. Thermal Sciences, v.158 p.106529, Full Text
  • Şahin, M.A., Çetin, B., Özer, M.B. (2020). "Investigation of effect of design and operating parameters on acoustophoretic particle separation via 3D device-level simulations", Microfluid. Nanofluid., v.24(8) p.1-18, Full Text
  • Rasooli, R., Çetin, B. (2019). "An extended Langhaar's solution for two-dimensional entry microchannel flows with high-order slip", Mathematics Applied to Engineering, Modelling, and Social Issues (Editors: Frank Smith, Hemen Dutta and John N. Mordeson), Springer p.189-212, Full Text
  • Çelebi, A.T., Çetin, B., Beşkök, A. (2019). "Molecular and Continuum Perspectives on Intermediate and Flow Reversal Regimes in Electroosmotic Transport", J. Physical Chemistry C, v.123(22) p.14024-14035, Full Text
  • Düven, G., Çetin, B., Kurtuldu, H., Gündüz, G.T., Tavman, Ş., Kışla, D. (2019). "A portable microfluidic platform for rapid determination of microbial load and somatic cell count", Biomed. Microdev., v.21(3) p.49, Full Text
  • Kibar, G., Çalışkan, U., Erdem, Y., Çetin, B. (2019). "One-pot synthesis of polyhedral oligomeric silsesquiox- ane (POSS) microparticles in a microfluidic chip", Full Text
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  • Akkuş, Y., Çetin, B., Dursunkaya, Z. (2019). "An iterative solution approach to coupled heat and mass transfer in a steadily fed evaporating water droplet", J. Heat Transfer, v.141(2) p.031501, Full Text
  • Alijani, H., Çetin, B., Akkus, Y., Dursunkaya, Z. (2019). "Experimental thermal performance characterization of flat grooved heat pipes", Heat Transfer Engineering, v.40(9-10) p.784–793, Full Text
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  • Yıldıran, İ.N., Temizer, İ., Çetin, B. (2017). "Homogenization in Hydrodynamic Lubrication: Microscopic Regimes and Re-Entrant Textures", Journal of Tribology, v.140 p.011701 (1-19), Full Text, DOI: http://tribology.asmedigitalcollection.asme.org/article.aspx?articleid=2628741
  • Çetin, B., Güler, K.G., Aksel, M.H. (2017). "Computational modeling of vehicle radiators using porous medium approach", Heat Exchangers -- Design, Experiment and Simulation (Edited by Prof. S. M. Sohel Murshed, Prof. M. L. S. Matos Lopes) InTech p.243–262, Full Text
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  • Çetin, B., Özer, M.B., Çağatay, E., Büyükkoçak, S. (2016). "An integrated acoustic and dielectrophoretic particle manipulation in a microfluidic device for particle wash and separation fabricated by mechanical machining", Biomicrofluidics, v.10 p.014112, Full Text, DOI: http://dx.doi.org/http://dx.doi.org/10.1063/1.4940431
  • Yavuz, C., Oliaei, S.N., Çetin, B., Yeşil-Çeliktaş, Ö. (2016). "Sterilization of PMMA microfluidic chips by various techniques and investigation of material characteristics", J. Supercrticial Fluids, v.107 p.114–121, Full Text, DOI: http://dx.doi.org/10.1016/j.supflu.2015.08.019
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  • Yavuz, C., Çetin, B., Yeşil-Çeliktaş, Ö. (2016). "Mikroakışkan Sistemlerin Sterilizasyonu", Mikroakışkan Teknolojilerin Temelleri ve Uygulamaları (Edited by Assoc. Prof. Ö. Yeşil-Çeliktaş) p.115–137
  • Yeşil-Çeliktaş, Ö., Çetin, B., Yıldırım, E. (2016). "Mikroakışkan Sistemlere Yönelik Gelecek Tahminleri", Mikroakışkan Teknolojilerin Temelleri ve Uygulamaları (Edited by Assoc. Prof. Ö. Yeşil-Çeliktaş) p.199–204
  • Zeinali, S., Çetin, B., Oliaei, S., Karpat, Y. (2015). "Fabrication of continuous flow microfluidics device with 3D electrode structures for high throughput DEP applications using mechanical machining", Electrophoresis, Special Issue on Dielectrophoresis, v.36(13) p.1432-1442, Full Text, DOI: http://dx.doi.org/10.1002/elps.201400486
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  • Karakaya, Z., Baranoğlu, B., Çetin, B., Yazıcı, A. (2015). "A parallel boundary element formulation for tracking multiple particle trajectories in Stoke's flow for microfluidic applications", CMES-Computer Modeling in Engineering and Science, v.104(3) p.227-249, Full Text
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  • Çetin, B., Zeinali, S. (2014). "Analysis of heat transfer for a low Peclet number microtube flow using second-order slip model: An extended-Graetz problem", J. Engineering Mathematics, v.89(1) p.13-25, Full Text
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  • Çetin, B., Aşık, M.D., Taze, S. (2014). "Design and fabrication of a microfluidic device for synthesis of chitosan nanoparticles", ASME J. Nanotechnology in Engineering and Medicine, v.4(3) p.031004, Full Text
  • Çetin, B. (2013). "Effect of thermal creep on heat transfer for a 2D microchannel flow: An analytical approach", ASME J. Heat Transfer, Special Issue on Microchannel Heat Transfer, v.135(10) p.101007, Full Text
  • Çetin, B., Li, D. (2011). "Dielectrophoresis in microfluidics technology", Electrophoresis, Special Issue on Dielectrophoresis, v.32 p.2410–2427
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  • Çetin, B., Cole, K. (2011). "The effect of axial conduction on heat transfer in a liquid microchannel flow", Int. J. Heat and Mass Transfer, v.54 p.2542-2549, DOI: http://dx.doi.org/10.1016/j.ijheatmasstransfer.2011.02.007
  • Çetin, B., Li, D. (2010). "Lab-on-a-chip device for continuous particle and cell separation based on electrical properties via AC-dielectrophoresis", Electrophoresis, v.31 p.3035-3043, DOI: http://dx.doi.org/10.1002/elps.20090007830
  • Çetin, B., Yazıcıoğlu, A.G., Kakaç, S. (2009). "Slip-flow heat transfer in microtubes with axial conduction and viscous dissipation-an extended Graetz problem", Int. J. Thermal Sciences, v.48 p.1673-1678, DOI: http://dx.doi.org/10.1016/j.ijthermalsci.2009.02.002
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  • Çetin, B., Li, D. (2009). "Continuous particle separation based on electrical properties using AC-DEP", Electrophoresis, v.30 p.3124-3133, DOI: http://dx.doi.org/10.1002/elps.20090007830
  • Çetin, B., Yazıcıoğlu, A.G., Kakaç, S. (2008). "Fluid Flow in microtubes with axial conduction including rarefaction and viscous dissipation", Int. Comm. Heat and Mass Transfer, v.35 p.535-544, DOI: http://dx.doi.org/10.1016/j.icheatmasstransfer.2008.01.003
  • Çetin, B., Li, D. (2008). "Effect of Joule heating on electrokinetic transport", Electrophoresis, v.29(5) p.994-1005, DOI: http://dx.doi.org/10.1002/elps.200700601
  • Çetin, B., Travis, B.E., Li, D. (2008). "Analysis of the electro-viscous effects on pressure-driven liquid flow in a two-section heterogeneous microchannel", Electrochimica Acta, v.54 p.660-664, DOI: http://dx.doi.org/10.1016/j.electacta.2008.07.008
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  • Çetin, B., Li, D. (2008). "Microfluidic Rotary Pump", Encyclopedia of Micro and Nanofluidics (1st Edition), Springer p.1188-1189, Full Text
  • Çetin, B., Li, D. (2008). "Temperature Gradient Generation and Control", Encyclopedia of Micro and Nanofluidics (1st Edition), Springer p.1993-1994, Full Text
  • Çetin, B., Li, D. (2008). "Methods for Pressure Measurements", Encyclopedia of Micro and Nanofluidics (1st Edition), Springer p.1743-1745, Full Text
  • Çetin, B., Li, D. (2008). "Magnetic Pumps", Encyclopedia of Micro and Nanofluidics (1st Edition), Springer p.1040-1043, Full Text
  • Çetin, B., Li, D. (2008). "Microfluidic Optical Devices", Encyclopedia of Micro and Nanofluidics (1st Edition), Springer p.1186-1187, Full Text
  • Çetin, B., Li, D. (2008). "Ultrasonic pumps", Encyclopedia of Micro and Nanofluidics (1st Edition), Springer p.2128-2129, Full Text